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One-pot native chemical ligation by combination of two orthogonal thioester precursors
Yuya Asahina1, Toru Kawakami1, Hironobu Hojo1
1Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan. hojo@protein.osaka-u.ac.jp.
Chemical Communications (Cambridge, England)
|January 31, 2017
Abstract:
We developed a one-pot peptide ligation method using two orthogonal thioester precursors and a protecting group for the ligation reaction between Asp and Cys. Combination of the two precursors facilitated the one-pot operation and yielded the entire polypeptide. The usefulness of this method was successfully demonstrated by the total synthesis of histone H4.
