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Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging
Published on: July 11, 2025
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A comparative study of ex vivo skin optical clearing using two-photon microscopy
Anton Sdobnov1, Maxim E Darvin2, Juergen Lademann2
1Research-Education Institute of Optics and Biophotonics, Saratov National Research State University, Astrakhanskaya 83, 410012, Saratov, Russian Federation.
Journal of Biophotonics
|January 31, 2017
Summary
Optical clearing agents like glycerol and Omnipaque improve skin imaging depth and contrast using multiphoton tomography (MPT). Omnipaque offers better safety and multimodal imaging potential compared to glycerol.
Area of Science:
- Biomedical Optics
- Dermatology
- Medical Imaging
Background:
- Multiphoton tomography (MPT) is a promising technique for skin imaging.
- Increasing MPT probing depth is crucial for detailed skin structure analysis.
Purpose of the Study:
- To compare the efficacy of glycerol and iohexol (Omnipaque) as optical clearing agents (OCAs) for ex vivo porcine ear skin.
- To evaluate the impact of OCA concentration and application time on MPT signal enhancement.
Main Methods:
- Ex vivo study on porcine ear skin using glycerol and Omnipaque at various concentrations.
- Topical application of OCAs for 60 minutes.
- Assessment of autofluorescence and second harmonic generation (SHG) signals via MPT.
Main Results:
- Both glycerol and Omnipaque significantly improved MPT signal depth and contrast.
- Glycerol showed higher effectiveness at applied concentrations but caused tissue shrinkage.
- Omnipaque demonstrated minimal tissue shrinkage and enhanced safety.
- Omnipaque enabled multimodal optical/X-ray imaging.
Conclusions:
- Optical clearing agents enhance MPT imaging of skin.
- Omnipaque is a safer and more versatile OCA than glycerol for specific applications, particularly multimodal imaging.
- Further research into Omnipaque for optical clearing in skin imaging is warranted.
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