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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Mar 8, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

Microarray Technology Applied to Human Allergic Disease.

Robert G Hamilton¹

¹Division of Allergy and Clinical Immunology, Departments of Medicine and Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA. rhamilt2@jhmi.edu.

Microarrays (Basel, Switzerland)

|January 31, 2017

Summary

This summary is machine-generated.

Microarray assays detect allergen-specific IgE antibodies for allergy diagnosis. While less sensitive than singleplex tests, they are valuable for research into population sensitization profiles.

Keywords:

ISAC IgE allergen extract component resolved diagnosis human immunoenzymetric assay immunosorbent allergen chip microarray molecular allergen serodiagnosis

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Area of Science:

Immunology
Allergy diagnostics
Biotechnology

Background:

Immunoglobulin E (IgE) antibodies mediate allergic reactions by triggering mast cells and basophils.
Early microarray assays utilized chip technology with purified allergens to detect IgE.
These assays enable simultaneous analysis of multiple allergen-specific IgE antibodies.

Purpose of the Study:

To describe the development and application of microarray-based IgE antibody assays.
To evaluate the utility of microarray assays in identifying patient sensitization profiles.
To compare the performance of microarray assays with singleplex methods.

Main Methods:

Immobilization of over 100 allergenic molecules on glass slides.
Detection of bound human IgE and IgG antibodies using fluorescently labeled anti-human IgE.
Analysis of fluorescent profiles to determine allergen-specific IgE profiles, including cross-reactivity.

Main Results:

Microarray assays can identify IgE antibodies binding to single or multiple allergens, including cross-reactive families.
The assay provides a sensitization profile for allergic patients.
Microarray assays are less sensitive and quantitative than singleplex assays like ImmunoCAP and Immulite.

Conclusions:

Chip-based microarray assays are effective research tools for defining population sensitization profiles in epidemiological studies.
Despite limitations in sensitivity and quantification compared to singleplex assays, microarrays offer a high-throughput method for analyzing IgE specificities.
Microarray technology has advanced allergy diagnostics by enabling comprehensive analysis of allergen-specific IgE.