Building ProteomeTools based on a complete synthetic human proteome

Area of Science:

• Proteomics
• Biomedical Research Tools
• Molecular Biology

Background:

• The human proteome is a complex biological system crucial for understanding health and disease.
• Developing comprehensive molecular and digital tools is essential for advancing biomedical research.
• Existing resources for human proteome analysis require expansion to cover all gene products comprehensively.

Purpose of the Study:

• To introduce ProteomeTools, a project creating molecular and digital tools from the human proteome.
• To report the generation and analysis of a large-scale synthetic human tryptic peptide library.
• To demonstrate the utility of this resource in various biomedical research applications.

Main Methods:

• Generation of over 330,000 synthetic tryptic peptides representing canonical human gene products.
• Multimodal liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of the synthetic peptides.
• Data curation and availability through a dedicated online resource (http://www.proteometools.org).

Main Results:

• Successfully synthesized and analyzed >330,000 unique synthetic tryptic peptides.
• These peptides cover essentially all canonical human gene products.
• Demonstrated the practical utility of the generated data through several application examples.

Conclusions:

• ProteomeTools provides a valuable, large-scale resource for the biomedical research community.
• The synthetic peptide library and associated data facilitate proteomic studies.
• The resource will be expanded and data will be continuously shared via ProteomicsDB and ProteomeXchange.