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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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Building ProteomeTools based on a complete synthetic human proteome.

Daniel P Zolg1, Mathias Wilhelm1, Karsten Schnatbaum2

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|January 31, 2017
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Summary

ProteomeTools generated over 330,000 synthetic human peptides for biomedical research. This extensive human proteome resource, including mass spectrometry data, is now publicly available to advance scientific discovery.

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Area of Science:

  • Proteomics
  • Biomedical Research Tools
  • Molecular Biology

Background:

  • The human proteome is a complex biological system crucial for understanding health and disease.
  • Developing comprehensive molecular and digital tools is essential for advancing biomedical research.
  • Existing resources for human proteome analysis require expansion to cover all gene products comprehensively.

Purpose of the Study:

  • To introduce ProteomeTools, a project creating molecular and digital tools from the human proteome.
  • To report the generation and analysis of a large-scale synthetic human tryptic peptide library.
  • To demonstrate the utility of this resource in various biomedical research applications.

Main Methods:

  • Generation of over 330,000 synthetic tryptic peptides representing canonical human gene products.
  • Multimodal liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of the synthetic peptides.
  • Data curation and availability through a dedicated online resource (http://www.proteometools.org).

Main Results:

  • Successfully synthesized and analyzed >330,000 unique synthetic tryptic peptides.
  • These peptides cover essentially all canonical human gene products.
  • Demonstrated the practical utility of the generated data through several application examples.

Conclusions:

  • ProteomeTools provides a valuable, large-scale resource for the biomedical research community.
  • The synthetic peptide library and associated data facilitate proteomic studies.
  • The resource will be expanded and data will be continuously shared via ProteomicsDB and ProteomeXchange.