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Global Proteome Changes in the Rat Diaphragm Induced by Endurance Exercise Training
Kurt J Sollanek1, Jatin G Burniston2, Andreas N Kavazis3
1Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, Florida, United States of America.
Plos One
|January 31, 2017
Summary
Endurance exercise training protects diaphragm muscles from ventilator-induced atrophy by altering key cytoprotective proteins. This study identified specific proteins that may be responsible for this exercise-induced protection in diaphragm muscle.
Area of Science:
- Physiology
- Biochemistry
- Exercise Science
Background:
- Mechanical ventilation (MV) is critical for critically ill patients but can cause diaphragmatic atrophy and weakness.
- Endurance exercise training confers protection against this ventilator-induced diaphragmatic atrophy, but the underlying mechanisms are unknown.
Purpose of the Study:
- To investigate exercise-induced changes in diaphragm muscle proteins.
- To compare the diaphragmatic proteome of sedentary and exercise-trained rats to identify protective mechanisms against ventilator-induced atrophy.
Main Methods:
- Proteomics analysis using label-free liquid chromatography-mass spectrometry.
- Profiling of soluble and mitochondrial proteins from rat diaphragm muscle.
- Comparison of protein abundance between sedentary and endurance-trained groups.
Main Results:
- Endurance exercise significantly altered the abundance of 70 soluble and 25 mitochondrial diaphragm proteins.
- Key cytoprotective proteins, including Mtfp1, 3MPST, Mgst3, and HSP70, increased in abundance after exercise training.
Conclusions:
- Endurance exercise training induces significant changes in the diaphragm proteome, increasing the abundance of potentially cytoprotective proteins.
- These identified proteins represent novel targets for future research into preventing ventilator-induced diaphragmatic atrophy.
