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Normal human cell proteins that interact with the adenovirus type 5 E1B 55kDa protein

George Hung1, S J Flint1

  • 1Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.

Virology
|January 31, 2017
PubMed

Insights

Researchers identified over 90 proteins associated with the human adenovirus type 5 E1B 55kDa protein, revealing new insights into viral infection mechanisms and host cell interactions. This study enhances understanding of adenovirus E1B 55kDa protein functions.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • The human adenovirus type 5 (HAdV-C5) E1B 55kDa protein plays crucial roles in viral infection, partly through a viral E3 ubiquitin ligase complex.
  • However, key substrates of this ligase and functions independent of it remain largely unknown.

Purpose of the Study:

  • To identify proteins specifically associated with the HAdV-C5 E1B 55kDa protein.
  • To characterize the cellular interactome of the E1B 55kDa protein and understand its role in viral replication and host cell modulation.

Main Methods:

  • Immunoaffinity purification of E1B 55kDa-associated proteins from infected human cells.
  • Liquid chromatography-mass spectrometry (LC-MS) analysis to identify and quantify bound proteins.
  • Infection of cells with wild-type HAdV-C5 and E1B 55kDa protein-null mutant viruses.

Main Results:

  • Identification of over 90 E1B 55kDa-associated proteins, including previously known interactors.
  • Enrichment of identified proteins in pathways related to the ubiquitin-proteasome system, RNA metabolism, and the cell cycle.
  • Demonstration of severe inhibition of viral genome replication upon siRNA and interferon treatment prior to infection.

Conclusions:

  • The E1B 55kDa protein interacts with a broad range of cellular proteins involved in fundamental cellular processes.
  • These findings provide a comprehensive protein interaction map for the E1B 55kDa protein, aiding in understanding its multifaceted roles during HAdV-C5 infection.
  • The study highlights the importance of host-pathogen interactions and potential therapeutic targets in adenovirus infections.
Keywords:
E1B 55kDa proteinHuman adenovirus type 5 (HAdV-C5)Mass spectrometryNormal human cellsRNA metabolismType I interferonsUbiquitin-proteasome system

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