Translocation of ¹³³Cs administered to Cryptomeria japonica wood

To reveal the in planta behaviour of caesium (Cs), the stable isotope ¹³³Cs was administered into 3-year-old Cryptomeria japonica seedlings by the application of ¹³³CsCl aqueous solution to the bark surface. The administered ¹³³Cs was quantified by ICP-MS measurements, which showed transportation of ¹³³Cs in an ascending direction in the stem. Distribution of ¹³³Cs was visualized using freeze-fixed C. japonica woody stem samples and cryo-time-of-flight secondary ion mass spectrometry/scanning electron microscopy (cryo-TOF-SIMS/SEM) analysis. Cryo-TOF-SIMS/SEM visualization suggested that ¹³³Cs was rapidly transported radially by ray parenchyma cells followed by axial transportation by pith and axial parenchyma cells. Adsorption experiments using powdered C. japonica wood samples and X-ray absorption fine structure (XAFS) analysis suggested that ¹³³Cs was in the hydrated state following its deposition into tracheid cell walls.