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siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
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MicroRNA-141 Targets Sirt1 and Inhibits Autophagy to Reduce HBV Replication

Ying Yang, Yanning Liu, Jihua Xue

    Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
    |January 31, 2017
    PubMed

    Insights

    MicroRNA-141 (miR-141) inhibits hepatitis B virus (HBV) replication by targeting Sirtuin 1 (Sirt1) and regulating autophagy. This finding offers potential therapeutic targets for treating chronic HBV infection.

    Area of Science:

    • Hepatology
    • Molecular Biology
    • Virology

    Background:

    • Chronic hepatitis B virus (HBV) infection affects 400 million people globally, increasing the risk of liver cirrhosis and hepatocellular carcinoma.
    • Recent research indicates a link between HBV replication and hepatocyte autophagy.
    • The specific role of microRNA-141 (miR-141) in this interaction requires further investigation.

    Purpose of the Study:

    • To investigate the function of miR-141 in modulating autophagy.
    • To determine the effect of miR-141 on hepatitis B virus replication.
    • To elucidate the regulatory mechanism of miR-141 in hepatocytes.

    Main Methods:

    • Quantitative real-time PCR was used to measure HBV-DNA, miR-141, and Sirt1 mRNA expression.
    • ELISA and Western blotting were employed to assess HBsAg, HBeAg, and protein levels.
    • Immunofluorescence and dual-luciferase reporter assays were utilized to analyze autophagy and miR-141/Sirt1 interactions.

    Main Results:

    • miR-141 mimic suppressed autophagy, HBV replication, and Sirt1 expression in hepatocytes.
    • miR-141 inhibitor increased autophagy and Sirt1 expression.
    • Bioinformatic analysis and luciferase assays confirmed Sirt1 as a direct target of miR-141.

    Conclusions:

    • miR-141 plays a crucial role in inhibiting HBV replication through autophagy regulation by targeting Sirt1.
    • This study identifies a novel regulatory pathway with potential implications for developing new anti-HBV therapies.
    Abstract

    Keywords:
    AutophagyHBVSirt1miR-141

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