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Related Experiment Videos

Case report: when two and ½ men go camping….

Matthias von Rotz1,2, Alexa Dierig3, Ulrich Heininger3

  • 1Division of Infectious Diseases and Hospital Epidemiology, University Hospital Basel, Basel, Switzerland.

BMC Infectious Diseases
|February 1, 2017
PubMed
Summary

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This summary is machine-generated.

Rapid identification of bacterial species from blood cultures using MALDI-TOF MS aids early treatment adjustments. This method quickly identified Listeria monocytogenes in a father and son sepsis case.

Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Infectious Diseases

Background:

  • Timely bacterial identification in patients is crucial for effective early-stage treatment guidance.
  • New protocols for direct identification from positive blood culture flasks offer significant advantages in clinical case management.

Observation:

  • Two patients, a father and son, presented with symptoms including fever, malaise, and diarrhea.
  • Blood cultures revealed short Gram-positive rods, prompting further investigation.
  • MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time of Flight) mass spectrometry was employed for rapid identification.

Findings:

  • The MALDI-TOF protocol directly from positive blood cultures rapidly identified Listeria monocytogenes as the causative agent of sepsis.
Keywords:
Listeria monocytogenesMALDI-TOFPulsed field gel electrophoresisRapid identificationSepsisTyping

Related Experiment Videos

  • Conventional methods, such as VITEK 2, confirmed the identification of Listeria monocytogenes 24 hours later.
  • Early antibiotic treatment adjustments were made based on the MALDI-TOF results, improving patient management.
  • Pulsed field gel electrophoresis confirmed the genetic relatedness of the Listeria monocytogenes isolates from the father and son.

    • Implications:

    • MALDI-TOF based identification directly from positive blood cultures can significantly expedite the diagnosis of bacterial sepsis.
    • Rapid diagnostic capabilities enable prompt and targeted antibiotic therapy, potentially improving patient outcomes.
    • This approach streamlines the diagnostic workflow, reducing the time to definitive identification compared to conventional methods.