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Updated: Mar 8, 2026
Electrophoretic Separation of Proteins
Published on: June 12, 2008
An innovative ring-shaped electroeluter for high concentration preparative isolation of protein from polyacrylamide
Jing-Jing Chen1, Guo-Qing Li1, Amit Pratush1
1Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Abstract:
A ring-shaped electroeluter (RSE) was designed for protein recovery from polyacrylamide gel matrix. The RSE was designed in such a way that a ring-shaped well was used to place gel slices and an enrichment well was used to collect eluted protein samples. With HSA as model protein, the electroelution time was less than 30 min with 80% recovery rate, and the concentration of recovered protein was 50 times higher than that of conventional method. The RSE could be reused at least ten times. The developed device makes great advance towards economic electroelution of biomolecules (such as proteins) from gel matrix.
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