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Systematic Chemoenzymatic Synthesis of O-Sulfated Sialyl Lewis x Antigens
Abhishek Santra1, Hai Yu1, Nova Tasnima1
1Department of Chemistry, University of California, Davis One Shields Avenue, Davis, CA 95616 (USA) xiichen@ucdavis.edu.
Abstract:
O-Sulfated sialyl Lewis x antigens play important roles in nature. However, due to their structural complexity, they are not readily accessible by either chemical or enzymatic synthetic processes. Taking advantage of a bacterial sialyltransferase mutant that can catalyze the transfer of different sialic acid forms from the corresponding sugar nucleotide donors to Lewis x antigens which are fucosylated glycans as well as an efficient one-pot multienzyme (OPME) sialylation system, O-sulfated sialyl Lewis x antigens containing different sialic acid forms and O-sulfation at different locations were systematically synthesized by chemoenzymatic methods.
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