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Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia
Published on: June 28, 2018
Antibiotic resistance & pathogen profile in ventilator-associated pneumonia in a tertiary care hospital in India
Abhijit Chaudhury1, A Shobha Rani1, Usha Kalawat1
1Department of Microbiology, Sri Venkateswara Institute of Medical Sciences, Tirupati, India.
Background & Objectives:
Ventilator-associated pneumonia (VAP) is an important hospital-acquired infection with substantial mortality. Only a few studies are available from India addressing the microbiological aspects of VAP, which have been done with small study populations. This study was carried out in the intensive care units (ICUs) of a tertiary care hospital to assess the profile of pathogens and to determine the pattern of antimicrobial resistance.
Methods:
This was a retrospective study of clinically suspected cases of VAP. Over a three year period, a total of 247 cases in 2011, 297 in 2012 and 303 in 2013 admitted in ICUs on mechanical ventilation with clinical evidence of VAP were included in our study. The endotracheal aspirate samples from these suspected cases were subjected to quantitative culture technique, and colony count of ≥10[5] colony forming units/ml was considered significant. Antimicrobial susceptibility test for the isolates was done.
Results:
VAP rates of 44.1, 43.8 and 26.3 were seen in 2011, 2012 and 2013, respectively. In all the three years, non-fermentative Gram-negative bacilli were the predominant organisms, followed by Pseudomonas spp. and Klebsiella spp. Staphylococcus aureus exhibited a downwards trend in prevalence from 50.0 per cent in 2011 to 34.9 per cent in 2013. An increase in vancomycin-resistant enterococci was seen from 4.3 per cent in 2012 to 8.3 per cent in 2013, while methicillin resistance amongst the S. aureus crossed the 50 per cent mark in 2013. An increasing trend in resistance was shown by Pseudomonas spp. for piperacillin-tazobactam (PTZ), amikacin and imipenem (IPM). For the non-fermenters, resistance frequency remained very high except for IPM (33.1%) and polymyxin-B (2.4%).
Interpretation & Conclusions:
Our findings show VAP as an important problem in the ICU setting. The incidence of multidrug-resistant pathogens was on the rise. The resistance pattern of these pathogens can help an institution to formulate effective antimicrobial policy. To have a comprehensive pan-India picture, multicentric studies are needed.
Insights
Ventilator-associated pneumonia (VAP) is a significant ICU infection in India. Rising multidrug-resistant pathogens necessitate updated antimicrobial policies based on local resistance patterns.
Area of Science:
- Critical Care Medicine
- Infectious Diseases
- Microbiology
Background:
- Ventilator-associated pneumonia (VAP) poses a significant threat in intensive care units (ICUs), contributing to substantial mortality.
- Limited data exists from India regarding the microbiological landscape and antimicrobial resistance patterns of VAP, particularly with small study cohorts.
Purpose of the Study:
- To investigate the microbiological profile of VAP cases in a tertiary care hospital ICU.
- To determine the antimicrobial resistance patterns of prevalent VAP pathogens over a three-year period.
Main Methods:
- Retrospective analysis of clinically suspected VAP cases admitted to ICUs on mechanical ventilation.
- Quantitative culture of endotracheal aspirates with a significant threshold of ≥10^5 CFU/ml.
- Antimicrobial susceptibility testing performed on isolated pathogens.
Main Results:
- VAP rates varied annually, with non-fermentative Gram-negative bacilli, Pseudomonas spp., and Klebsiella spp. as predominant organisms.
- Prevalence of Staphylococcus aureus decreased, but methicillin resistance exceeded 50% by 2013.
- Increasing resistance observed in Pseudomonas spp. to common antibiotics; high resistance rates noted for non-fermenters, except to imipenem and polymyxin-B.
Conclusions:
- VAP remains a critical issue in ICUs, characterized by an increasing incidence of multidrug-resistant pathogens.
- Understanding local antimicrobial resistance trends is crucial for developing effective institutional antimicrobial stewardship policies.
- Multicentric studies are essential to establish a comprehensive understanding of VAP epidemiology across India.
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