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Updated: Mar 8, 2026

Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells

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Published on: July 21, 2009

Improvements in cell block processing: The Cell-Gel method.

Kristin A La Fortune¹, Melissa L Randolph¹, Howard H Wu¹

¹Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana.

Cancer Cytopathology

|February 1, 2017

Summary

This summary is machine-generated.

The Cell-Gel method offers an effective, inexpensive way to produce abundant cell blocks for cytology diagnostics. This new protocol significantly reduced the cell block failure rate by approximately 67%.

Keywords:

clinical cytological technique cytology cytopathology fine-needle aspiration laboratory techniques

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Area of Science:

Cytopathology
Histopathology Techniques

Background:

Adequate cell block production is crucial for the diagnostic utility of cytology specimens.
Cell blocks are standard for fine-needle aspiration and fluid samples, often requiring dedicated passes for ancillary studies.

Purpose of the Study:

To introduce and evaluate the Cell-Gel method, an effective and inexpensive protocol for producing abundant cell blocks.
To compare the efficacy of the Cell-Gel method against the HistoGel Tube method in reducing cell block failure rates.

Main Methods:

The Cell-Gel method involves three steps: sample preparation using a hemolytic fixative, cell block construction with disposable base molds, and standard processing.
A comparative study evaluated 110 cell blocks processed with the HistoGel Tube method and 110 with the Cell-Gel method.

Main Results:

The Cell-Gel method demonstrated a significant reduction in cell block failure rate, decreasing from 18% (HistoGel Tube) to 6% (Cell-Gel).
This represents an approximate 67% decrease in the failure rate for the study laboratory.

Conclusions:

The Cell-Gel method is an effective and economical protocol for generating adequate cell blocks with abundant cellular material.
The protocol's highlights include the use of a hemolytic fixative and disposable base molds, leading to improved diagnostic specimen quality.