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Measuring high-sensitivity cardiac troponin T blood concentration in population surveys

Antonio Ivan Lazzarino1,2, Jennifer S Mindell2

  • 1London School of Hygiene & Tropical Medicine, London, United Kingdom.

Plos One
|February 1, 2017
PubMed

Insights

High-sensitivity cardiac troponin T (HS-CTnT) blood tests are feasible for large population surveys. Stored samples showed reliable cardiovascular risk marker results, supporting their use in national health assessments.

Area of Science:

  • Cardiovascular Disease Biomarkers
  • Population Health Surveys
  • Clinical Chemistry

Background:

  • High-sensitivity cardiac troponin T (HS-CTnT) is a proposed cardiovascular risk marker in the general population.
  • HS-CTnT levels correlate with future cardiovascular events and mortality.
  • Feasibility of HS-CTnT testing in large, nationally-representative surveys needs evaluation.

Purpose of the Study:

  • To assess the feasibility of measuring HS-CTnT in samples collected during household visits.
  • To determine if standard postal shipping and freezing affect HS-CTnT test results.
  • To evaluate the test-retest reliability of HS-CTnT measurements after storage.

Main Methods:

  • Utilized blood samples from 200 participants in the Health Survey for England (HSE) 2016.
  • Stored samples at -40°C for 5-10 weeks before retesting.
  • Analyzed within-person agreement using Cronbach's Alpha and Interclass Correlation Coefficient.

Main Results:

  • High reliability was observed, with Cronbach's Alpha at 0.97 and ICC at 0.95.
  • No significant impact on results was found from the delay (1-4 days) between blood withdrawal and storage.
  • Freezing time (5-10 weeks) did not affect the accuracy of HS-CTnT measurements.

Conclusions:

  • Measuring HS-CTnT plasma concentration is feasible within large, nationally-representative surveys like the HSE.
  • Standard sample handling, shipping, and storage protocols are compatible with reliable HS-CTnT testing.
  • This supports the integration of HS-CTnT testing into routine population health surveillance for cardiovascular risk assessment.
Abstract

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