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Assessment of Avenins from Different Oat Varieties Using R5-Based Sandwich ELISA.

Lora Benoit^1,2, Jongkit Masiri³, Isabel A Del Blanco⁴

¹IEH Laboratories and Consulting Group, Inc. (IEH) , 15300 Bothell Way N.E., Lake Forest Park, Washington 98155, United States.

Journal of Agricultural and Food Chemistry

|February 1, 2017

Summary

This summary is machine-generated.

Some oats contain gluten that can trigger celiac disease (CD) in susceptible individuals. This study found specific oat varieties exceed the 20 ppm gluten threshold, posing risks for CD patients and labeling regulations.

Keywords:

ELISA R5 monoclonal antibody avenin celiac disease gluten oats prolamins

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Area of Science:

Food science
Immunology
Analytical chemistry

Background:

Wheat-derived gluten is immunopathogenic in celiac disease (CD).
The role of oat avenins in CD pathogenesis is debated, impacting gluten-free labeling laws.
The R5 monoclonal antibody (mAb) detects epitopes in wheat gluten but is reportedly absent in avenins.

Purpose of the Study:

To investigate the presence of gluten in pure oat cultivars using the R5 mAb.
To determine if specific oat varieties exceed the regulatory gluten threshold for gluten-free labeling.

Main Methods:

Sandwich ELISA using the R5 mAb to analyze oat cultivars.
Nested assessment with Western blot and other gluten detection systems to corroborate ELISA results.

Main Results:

Three oat varieties showed reproducible R5 mAb binding.
Gluten levels in these varieties exceeded the 20 ppm regulatory threshold.
Western blot and alternative methods confirmed the presence of gluten.

Conclusions:

Select oat varieties contain gluten that can be detected by R5 mAb and exceed regulatory limits.
These findings have implications for celiac disease patients, food companies, and regulatory agencies.
The study enhances understanding of gluten detection systems and oat safety for celiacs.