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Feedback Regulation of Calcium Concentration

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...

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Related Experiment Video

Updated: Mar 8, 2026

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Published on: May 25, 2011

Exploring the Ca²⁺-dependent synaptic dynamics in vibro-dissociated cells.

Ulyana Lalo¹, Yuriy Pankratov²

¹The University of Warwick, School of Life Sciences, Coventry, UK.

|February 2, 2017

Summary

This study introduces non-enzymatic vibro-dissociation to isolate neurons, enabling detailed analysis of synaptic plasticity. Researchers observed calcium-dependent modulation of AMPA and NMDA receptors in individual synaptic boutons.

Keywords:

ATP receptor Astrocyte NMDA receptor Quantal synaptic current Receptor trafficking Single-synapse response Synaptic strength Vesicular release

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Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

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Published on: July 15, 2012

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Last Updated: Mar 8, 2026

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

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Published on: May 25, 2011

Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals

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Published on: October 1, 2014

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Published on: July 15, 2012

Area of Science:

Neuroscience
Cell Biology
Synaptic Plasticity

Background:

Synaptic strength dynamic alteration is crucial for nervous system function.
Studying quantal synaptic currents and glial cell impact faces technical challenges.
Existing methods like cell culture can introduce artifacts.

Purpose of the Study:

To present non-enzymatic vibro-dissociation as a method to isolate functional synapses.
To investigate synaptic plasticity mechanisms at individual synaptic boutons.
To analyze calcium-dependent modulation of postsynaptic receptors.

Main Methods:

Non-enzymatic vibro-dissociation for neuron isolation.
Electrophysiology and confocal imaging.
Molecular biology techniques.

Main Results:

Isolated neurons preserved functional synapses without cell culture artifacts.
Detailed analysis of individual synaptic boutons was achieved.
Calcium-dependent modulation of postsynaptic AMPA and NMDA receptors was observed.

Conclusions:

Non-enzymatic vibro-dissociation is valuable for studying synaptic plasticity.
The method allows fine details of synaptic mechanisms to be resolved.
New insights into calcium's role in receptor modulation were gained.