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Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS

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Published on: May 3, 2011

Surface-enhanced infrared absorption studies towards a new optical biosensor.

Lothar Leidner¹, Julia Stäb¹, Jennifer T Adam¹

¹Institute of Physical and Theoretical Chemistry (IPTC), Eberhard Karls University of Tübingen, Auf der Morgenstelle 18, 72076 Tübingen, Germany.

Beilstein Journal of Nanotechnology

|February 2, 2017

Summary

Surface-enhanced infrared absorption (SEIRA) magnifies mid-infrared spectra for sensors. This technique enhances weak absorption signals, improving analyte detection in reflectometric interference spectroscopy (RIfS).

Keywords:

attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR)direct optical sensing mid-infrared regime (MIR)reflectometric interference spectroscopy surface-enhanced infrared absorption (SEIRA)

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Area of Science:

Spectroscopy
Chemical Sensing
Surface Science

Background:

Reflectometric interference spectroscopy (RIfS) is established for optical thickness changes.
Mid-infrared spectroscopy offers additional information via absorption spectra.
Surface-enhanced infrared absorption (SEIRA) can magnify weak spectra.

Purpose of the Study:

To demonstrate SEIRA's ability to enhance weak mid-infrared absorption spectra.
To investigate the complex water band at 3300 cm⁻¹ using SEIRA.
To compare SEIRA-RIfS with attenuated total reflectance (ATR) spectroscopy.

Main Methods:

Utilizing reflectometric interference spectroscopy (RIfS) in the mid-infrared range.
Applying surface-enhanced infrared absorption (SEIRA) to enhance spectral signals.
Analyzing the broad water band (3300 cm⁻¹) influenced by H-bonds and Fermi resonance.
Comparing results with an attenuated total reflectance (ATR) setup.

Main Results:

SEIRA successfully magnified the weak absorption spectra of the water band.
The enhanced spectra provided detailed information about the water band's complex nature.
SEIRA-RIfS demonstrated potential for improved spectral resolution compared to ATR.

Conclusions:

SEIRA is a powerful technique for enhancing mid-infrared spectra in RIfS sensors.
This approach improves the detection and analysis of analytes through their vibrational fingerprints.
SEIRA-RIfS offers a promising alternative for sensitive chemical sensing applications.