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Updated: Mar 8, 2026

FLIM-FRET Measurements of Protein-Protein Interactions in Live Bacteria.
Published on: August 25, 2020
Comment on 'A novel method for fast and robust estimation of fluorescence decay dynamics using constrained
Yongliang Zhang1,2, David Day-Uei Li1
1Centre for Biophotonics, Strathclyde Institute of Pharmacy & Biomedical Sciences, University of Strathclyde, Glasgow, Scotland, UK.
None:
This comment is to clarify that Poisson noise instead of Gaussian noise shall be included to assess the performances of least-squares deconvolution with Laguerre expansion (LSD-LE) for analysing fluorescence lifetime imaging data obtained from time-resolved systems. Moreover, we also corrected an equation in the paper. As the LSD-LE method is rapid and has the potential to be widely applied not only for diagnostic but for wider bioimaging applications, it is desirable to have precise noise models and equations.
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