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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Published on: July 11, 2016

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Application of the MALDI-TOF for identification of Clostridium perfringens strains

Claudia Brodzik, Ewa Augustynowicz, Agnieszka Korzeniowska-Kowal

    Medycyna Doswiadczalna I Mikrobiologia
    |February 2, 2017
    PubMed
    Abstract

    Insights

    The MALDI-TOF method effectively identifies Clostridium perfringens, a common cause of food poisoning. This rapid identification aids in epidemiological investigations of foodborne illnesses.

    Area of Science:

    • Microbiology
    • Food Safety
    • Diagnostic Technology

    Background:

    • Clostridium perfringens is an anaerobic bacterium found in soil, sewage, and animal/human gastrointestinal tracts.
    • C. perfringens is a leading cause of foodborne illness globally.
    • Understanding C. perfringens toxinogenesis, transmission, and diagnostics is crucial for public health.

    Purpose of the Study:

    • To evaluate the suitability of the MALDI-TOF mass spectrometry method for identifying Clostridium perfringens species.

    Main Methods:

    • MALDI-TOF mass spectrometry was employed for bacterial identification.
    • 16S rRNA gene sequencing was used for verification.
    • Duplex PCR was utilized for the detection of cpe and cpa genes.

    Main Results:

    • MALDI-TOF successfully identified C. perfringens in 39 out of 41 archival isolates.
    • 16S rRNA sequencing and cpa gene detection confirmed the accuracy of MALDI-TOF results.

    Conclusions:

    • The MALDI-TOF method is a useful tool for the rapid identification of anaerobic bacteria, specifically C. perfringens.
    • This rapid identification capability supports its application in epidemiological studies of foodborne diseases.

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