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Updated: Mar 8, 2026

High-throughput Screening for Protein-based Inheritance in S. cerevisiae
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Genetic Methods for Studying Yeast Prions.

Reed B Wickner1, Herman K Edskes2, Dmitry Kryndushkin3

  • 1Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0830; wickner@helix.nih.gov.

Cold Spring Harbor Protocols
|February 3, 2017
PubMed
Summary
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This study details methods for identifying yeast prions, focusing on their unique genetic traits rather than observable characteristics. It outlines assays and genetic techniques crucial for prion research.

Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • Prions are proteins that can adopt abnormal conformations, leading to disease.
  • Certain nonchromosomal genetic elements were reclassified as prions based on genetic, not phenotypic, properties.

Purpose of the Study:

  • To outline methods for identifying and studying yeast prions.
  • To differentiate prion-based traits from other nonchromosomal genetic elements.

Main Methods:

  • Prion assay development.
  • Demonstration of nonchromosomal inheritance.
  • Characterization of prion-specific genetic properties.
  • Application of genetic methods for yeast prion studies.

Main Results:

  • Established methodologies for prion identification in yeast.

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  • Distinguished prion characteristics from other nonchromosomal genetic elements.
  • Provided a framework for genetic analysis of yeast prions.

    • Conclusions:

    • Yeast prion identification relies on unique genetic properties.
    • Specific assays and genetic strategies are essential for studying yeast prions.
    • This work facilitates further research into prion biology and genetics.