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Updated: Mar 8, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
Gene Specific Impedimetric Bacterial DNA Sensor for Rheumatic Heart Disease
Swati Singh1,2, Ankur Kaushal1, Sunil Gupta3
1CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007 India.
Abstract:
An impedimetric mga gene specific DNA sensor was developed by immobilization of single stranded DNA probe onto the screen printed modified gold-dendrimer nanohybrid composite electrode for early and rapid detection of S. pyogenes in human throat swab samples causing rheumatic heart disease. Electrochemical impedance response was measured after hybridization with bacterial single stranded genomic DNA (ssG-DNA) with probe. The sensor was found highly specific to S. pyogenes and can detect as low as 0.01 ng ssDNA in 6 µL sample only in 30 min. The nanohybrid sensor was also tested with non-specific pathogens and characterized by FTIR. An early detection of the pathogen S. pyogenes in human can save damage of mitral and aortic heart valves (rheumatic heart disease) by proper medical care.
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