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Breed and adaptive response modulate bovine peripheral blood cells' transcriptome.
Nataliya Pošćić1, Tommaso Montanari1, Mariasilvia D'Andrea2
1Department of Agriculture, Food, Environment and Animal Science (DI4A), University of Udine, via delle Scienze 206, 33100 Udine, Italy.
Journal of Animal Science and Biotechnology
|February 3, 2017
Summary
Cows with high levels of acute phase proteins (APPs) show distinct gene expression patterns related to adaptive responses. These patterns, particularly in Italian Holstein (IH) cows, are linked to metabolic activity and milk production variations.
Area of Science:
- Animal Genomics
- Transcriptomics
- Veterinary Medicine
Background:
- Adaptive responses involve physiological changes to environmental stimuli.
- Acute phase proteins (APPs) are key indicators of stress, infection, and inflammation in animals.
Purpose of the Study:
- To compare the molecular basis of adaptive responses in Italian Holstein (IH) and Italian Simmental (IS) cows.
- To identify differentially expressed genes (DEGs) and impacted pathways associated with high APP levels.
Main Methods:
- Whole-blood transcriptome analysis (RNA-seq) was performed on 663 IH and IS cows.
- Cows were grouped into APP-high (APP+) and APP-low (APP-) variants.
- Gene expression data were analyzed using Dynamic Impact Approach (DIA) and DAVID annotation.
Main Results:
- 1,152 DEGs were identified between all APP+ and APP- variants.
- Specific DEGs were found for IH (531) and IS (217) breeds.
- Key impacted pathways included vitamin B6 metabolism, folate biosynthesis, nitrogen metabolism, and linoleic acid metabolism.
Conclusions:
- DIA and DAVID analyses identified significant DEGs and pathways related to adaptive response in high-APP cows.
- Similar gene expression patterns were observed between IH and IS breeds, with IH showing more pronounced impacts.
- Gene expression variations were significantly associated with milk production parameters.
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