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Published on: March 3, 2023
Bloodstream infections and antimicrobial sensitivity patterns in a tertiary care hospital of India
Nikita Vasudeva1, Prem Singh Nirwan2, Preeti Shrivastava2
1Department of Microbiology, NIMS UNIVERSITY Shobha Nagar, Jaipur-Delhi Highway (NH-11C), Jaipur - 303121, Jaipur, Rajasthan, India.
Background:
Invasion of the bloodstream by microorganisms constitutes one of the most serious situations in infectious disease. Microorganisms present in circulating blood whether continuously, intermittently, or transiently are a threat to every organ in the body. Prevalence and antimicrobial susceptibility of microorganisms vary depending upon the geography and the use of antibiotics.
Methods:
A cross-sectional study to determine the prevalent organisms causing bloodstream infection was conducted. BACTEC BD 9050 system was used to identify the causative organism, and sub-cultures were done on MacConkey Agar and Blood Agar. Antibiotic susceptibility test (AST) was done using Kirby B Disk diffusion method.
Results:
A total of 170 patients were enrolled, and blood samples of 53 patients showed growth of organisms. Staphylococcus aureus was the most commonly isolated organism. Most of the Gram-positive cocci (GPC) were susceptible to vancomycin and linezolid. Most of the Gram-negative bacilli (GNB) showed sensitivity to cefoperazone/sulbactam followed by imipenem.
Insights
Staphylococcus aureus is the most common cause of bloodstream infections. Gram-positive bacteria were susceptible to vancomycin, while Gram-negative bacteria responded best to cefoperazone/sulbactam.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Clinical Diagnostics
Background:
- Bloodstream infections (BSI) pose a severe threat to patient health.
- Microbial presence in blood can impact all organs.
- Organism prevalence and antibiotic resistance vary geographically.
Purpose of the Study:
- To identify prevalent microorganisms causing bloodstream infections.
- To determine antimicrobial susceptibility patterns in a specific patient cohort.
Main Methods:
- Cross-sectional study design.
- Utilized the BACTEC BD 9050 system for organism identification.
- Performed antibiotic susceptibility testing (AST) via Kirby-B Disk diffusion.
Main Results:
- Out of 170 patients, 53 had positive blood cultures.
- Staphylococcus aureus was the most frequently isolated pathogen.
- Gram-positive cocci showed susceptibility to vancomycin and linezolid.
- Gram-negative bacilli were most sensitive to cefoperazone/sulbactam and imipenem.
Conclusions:
- Staphylococcus aureus is a primary cause of BSIs in the studied population.
- Effective antibiotic options include vancomycin for Gram-positives and cefoperazone/sulbactam for Gram-negatives.
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