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Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Published on: March 20, 2018
Drug-DNA complexation as the key factor in photosensitized thymine dimerization
M Consuelo Cuquerella1, Virginie Lhiaubet-Vallet1, Miguel A Miranda1
1Instituto Universitario Mixto de Tecnología Química UPV-CSIC, Universitat Politècnica de València, Avda de los Naranjos, s/n, 46022 Valencia, Spain. fbosca@itq.upv.es.
Abstract:
The crucial role of photosensitizer@DNA complexation in the formation of cyclobutane pyrimidine dimers (CPDs) has been demonstrated using femtosecond and nanosecond transient absorption and emission measurements in combination with in vitro DNA damage assays. This finding opens the door to re-evaluate the mechanisms involved in CPDs photosensitized by other chemicals.
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