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Related Experiment Video

Updated: Mar 8, 2026

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

Multifunctional Liposomes.

Bhawani Aryasomayajula¹, Giuseppina Salzano², Vladimir P Torchilin^3,4

¹Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, 360 Huntington Ave., Boston, MA, 02115, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 3, 2017

Summary

Liposomes are versatile nanocarriers for drug delivery and diagnostics. Advanced stimuli-sensitive liposomes offer targeted release of therapeutics and improved imaging capabilities for various medical applications.

Keywords:

Cell-penetrating Liposomes Long-circulating MMP-sensitive Magnetically sensitive Multifunctional Redox-sensitive Temperature-sensitive Tumor-targeting pH-sensitive

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Last Updated: Mar 8, 2026

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

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Published on: August 31, 2019

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

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Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Area of Science:

Nanotechnology
Biomedical Engineering
Pharmaceutical Sciences

Background:

Liposomes, developed in the 1960s, were initially for drug delivery.
They enable co-delivery of diverse agents and targeted delivery via moieties like antibodies.
Stimuli-sensitive liposomes represent a dynamic research area.

Purpose of the Study:

To explore the preparation methods for functional liposomes.
To highlight the role of stimuli-sensitive liposomes in targeted drug delivery.
To discuss liposome applications in diagnostics and imaging.

Main Methods:

Incorporation of targeting moieties (proteins, sugars, antibodies).
Design of stimuli-sensitive liposomes responding to pH, enzymes, temperature, redox, or magnetic fields.
Utilizing lipid-polymer conjugates for cell-penetrating peptide incorporation.

Main Results:

Stimuli-sensitive liposomes enable targeted release of active agents.
Liposomes can be functionalized for enhanced cell penetration.
Liposomes serve diagnostic purposes, including tumor imaging with fluorescent probes or reporter metals for gamma/MR imaging.

Conclusions:

Liposomes are adaptable nanoplatforms for advanced drug delivery and diagnostics.
Stimuli-responsive designs enhance therapeutic targeting and efficacy.
Liposomes offer significant potential in personalized medicine and advanced imaging techniques.