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Evaluation of an automated light transmission aggregometry
Li-Qin Ling1, Juan Liao2, Qian Niu2
1a West China School of Medicine/West China Hospital , Sichuan University , Chengdu , P.R. China.
Platelets
|February 3, 2017
Summary
A new automated analyzer (Sysmex CS-2100i) offers a reliable method for platelet function testing, correlating well with gold-standard aggregometry. It requires fewer platelets and provides faster results for clinical use.
Area of Science:
- Hematology
- Clinical Pathology
- Laboratory Medicine
Background:
- Light transmission aggregometry (LTA) is the established standard for assessing platelet function.
- However, LTA is often time-consuming and labor-intensive, limiting its routine clinical application.
- Automated methods are needed to improve efficiency and standardization in platelet function testing.
Purpose of the Study:
- To evaluate the performance of a new automated platelet aggregation method on the Sysmex CS-2100i analyzer for clinical use.
- To assess its repeatability, correlation with a reference aggregometer, and minimum platelet count requirements.
- To determine the optimal diluent (platelet-poor plasma or physiological saline) for different agonists.
Main Methods:
- The Sysmex CS-2100i analyzer was used to perform platelet aggregation tests with various agonists: adenosine diphosphate (ADP), arachidonic acid, collagen, epinephrine, and ristocetin.
- Repeatability was assessed, and results were correlated with those obtained from a reference Chrono-log Model 700 aggregometer.
- Platelet-rich plasma (PRP) was diluted with either platelet-poor plasma (PPP) or physiological saline (PS) to evaluate the impact of diluents and determine the threshold for platelet counts.
Main Results:
- The Sysmex CS-2100i demonstrated excellent repeatability and a strong correlation with the Chrono-log 700.
- The analyzer's threshold for platelet counts in PRP was found to be 80 × 109/L.
- PPP inhibited aggregation induced by ADP, arachidonic acid, collagen, and epinephrine, while PS inhibited ristocetin-induced aggregation, necessitating specific diluent choices based on the agonist used.
Conclusions:
- The Sysmex CS-2100i provides a highly standardized, repeatable, and accurate method for platelet function assessment.
- Its lower platelet count requirement, shorter turnaround time, and walk-away capability make it suitable for routine clinical use.
- Optimal diluent selection (PPP or PS) is crucial for accurate agonist-specific platelet aggregation results.
