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Related Experiment Video

Updated: Mar 8, 2026

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
07:40

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Published on: December 8, 2017

10.4K

Microstructured Surface Arrays for Injection of Zebrafish Larvae.

Felix Ellett1, Daniel Irimia1

  • 1Department of Surgery, BioMEMS Resource Center, Massachusetts General Hospital, Harvard Medical School, Shriners Burns Hospital , Boston, Massachusetts.

Zebrafish
|February 3, 2017
PubMed
Summary

Researchers developed a new silicone device to immobilize zebrafish larvae for precise microinjection. This tool aids in delivering treatments and other substances to specific tissues in developing zebrafish.

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Area of Science:

  • Developmental Biology
  • Zebrafish Research
  • Microinjection Techniques

Background:

  • Microinjection is crucial for zebrafish research, enabling the delivery of various substances to specific tissues.
  • Existing methods lack specialized devices for orienting hatching-stage zebrafish larvae for targeted microinjection.

Purpose of the Study:

  • To present a novel, reusable silicone device for immobilizing 2 days postfertilization zebrafish larvae.
  • To facilitate precise microinjection into multiple anatomical targets in various orientations (lateral, ventral, dorsal).

Main Methods:

  • Design and fabrication of a reusable silicone device with arrayed microstructures.
  • Utilizing the device to immobilize zebrafish larvae at the 2 days postfertilization stage.
  • Demonstrating microinjection precision using rhodamine dextran.
Keywords:
infectionlarvaemicroinjectionmicrostructuresxenotransplantation

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Main Results:

  • The device effectively immobilizes zebrafish larvae in lateral, ventral, and dorsal orientations.
  • Maximal access to target sites for microinjection was achieved.
  • Precise microinjection into multiple anatomical targets was successfully demonstrated.

Conclusions:

  • The developed silicone device is a valuable tool for improving the precision and accessibility of microinjection in zebrafish larvae.
  • This innovation supports advancements in zebrafish research, including treatment delivery and xenotransplantation studies.