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Updated: Mar 8, 2026
Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
Published on: January 17, 2012
Identification of calgranulin B interacting proteins and network analysis in gastrointestinal cancer cells
Kyung-Hee Kim1,2, Seung-Gu Yeo3, Byong Chul Yoo2
1Omics Core Laboratory, Research Institute, National Cancer Center, Goyang-si, Gyeonggi-do, Republic of Korea.
Abstract:
Calgranulin B is known to be involved in tumor development, but the underlying molecular mechanism is not clear. To gain insight into possible roles of calgranulin B, we screened for calgranulin B-interacting molecules in the SNU-484 gastric cancer and the SNU-81 colon cancer cells. Calgranulin B-interacting partners were identified by yeast two-hybrid and functional information was obtained by computational analysis. Most of the calgranulin B-interacting partners were involved in metabolic and cellular processes, and found to have molecular function of binding and catalytic activities. Interestingly, 46 molecules in the network of the calgranulin B-interacting proteins are known to be associated with cancer and FKBP2 was found to interact with calgranulin B in both SNU-484 and SNU-81 cells. Polyubiquitin-C encoded by UBC, which exhibited an interaction with calgranulin B, has been associated with various molecules of the extracellular space and plasma membrane identified in our screening, including Na-K-Cl cotransporter 1 and dystonin in SNU-484 cells, and ATPase subunit beta-1 in SNU-81 cells. Our data provide novel insight into the roles of calgranulin B of gastrointestinal cancer cells, and offer new clues suggesting calgranulin B acts as an effector molecule through which the cell can communicate with the tumor microenvironment via polyubiquitin-C.
Insights
Calgranulin B interacts with proteins involved in cancer, including FKBP2 and Polyubiquitin-C. This suggests Calgranulin B may mediate cancer cell communication with the tumor microenvironment.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Calgranulin B's role in tumor development is established, but its molecular mechanisms remain unclear.
- Understanding Calgranulin B's interactions is crucial for elucidating its function in cancer.
Purpose of the Study:
- To identify molecules interacting with Calgranulin B in gastric and colon cancer cells.
- To gain insight into the molecular mechanisms underlying Calgranulin B's involvement in cancer.
Main Methods:
- Yeast two-hybrid screening was employed to identify Calgranulin B-interacting proteins.
- Computational analysis was used to determine the functional roles of these interacting partners.
Main Results:
- Numerous Calgranulin B-interacting partners were identified, predominantly involved in metabolic and cellular processes with binding and catalytic activities.
- A network of 46 cancer-associated molecules was identified, including FKBP2, which interacted with Calgranulin B in both cell lines.
- Polyubiquitin-C (UBC) interacted with Calgranulin B and was linked to extracellular and plasma membrane proteins like Na-K-Cl cotransporter 1 and ATPase subunit beta-1.
Conclusions:
- Calgranulin B plays a significant role in gastrointestinal cancers.
- Calgranulin B may function as an effector molecule facilitating cancer cell communication with the tumor microenvironment via Polyubiquitin-C.
