Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

14.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
14.9K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.6K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

945
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
945

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Case 351.

Radiology·2026
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

Nature methods·2026
Same author

Endovascular thrombectomy for patients with large-core ischaemic stroke presenting up to 24 h after onset (ATLAS): a systematic review and individual patient data meta-analysis with central imaging adjudication.

Lancet (London, England)·2026
Same author

mGluR6 coordinates cone terminal targeting and synaptic layer assembly during human retinal development.

bioRxiv : the preprint server for biology·2026
Same author

"It Honestly Took Me About Two Years": Minoritized Students' Experiences in Navigating Pathways Between High School, College, and Health Science Graduate Programs.

Medical science educator·2026
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Mar 8, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K

Single-shot quantitative phase microscopy with color-multiplexed differential phase contrast (cDPC).

Zachary F Phillips1, Michael Chen2, Laura Waller1,2

  • 1Graduate Group in Applied Science and Technology, University of California, Berkeley, United States of America.

Plos One
|February 3, 2017
PubMed
Summary

We developed a new microscopy method using coded illumination to recover quantitative phase and amplitude from a single image. This technique enables high-speed imaging of biological samples like cells and C. elegans.

More Related Videos

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.1K
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

16.2K

Related Experiment Videos

Last Updated: Mar 8, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.1K
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

16.2K

Area of Science:

  • Biophysics
  • Optical Microscopy
  • Image Processing

Background:

  • Quantitative phase microscopy provides valuable information about transparent biological samples.
  • Traditional methods often require complex setups or multiple measurements.

Purpose of the Study:

  • To introduce a novel, cost-effective technique for single-shot quantitative phase and amplitude microscopy.
  • To enable high-speed imaging of unstained biological specimens.

Main Methods:

  • Utilized a modified commercial brightfield microscope with a 3D-printed condenser insert.
  • Implemented color-multiplexed Differential Phase Contrast (cDPC) with partially coherent illumination.
  • Achieved resolution enhancement up to 2x the objective numerical aperture (NA).

Main Results:

  • Successfully recovered quantitative phase and amplitude from single color images.
  • Demonstrated high-speed imaging at 50 frames per second (fps).
  • Visualized various in vitro cell samples and C. elegans in microfluidic channels.

Conclusions:

  • The cDPC method offers a practical and efficient approach for advanced microscopy.
  • This technique facilitates label-free imaging and analysis of dynamic biological processes.
  • The system's simplicity and speed make it suitable for diverse research applications.