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Published on: July 28, 2016
An ultrasensitive fluorescent probe for rapid determination of thiophenols
Lin Lin1, Xiangqiong Zeng2, Yuning Shen3
1State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing 210023, China; Green Chemical Engineering Technology Research Center, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.
Abstract:
An ultrasensitive fluorescent probe TPP was developed for the highly selective detection of thiophenols based on excited-state intramolecular proton transfer (ESIPT) mechanism, its synthesis through a simple straightforward combination of HBT fluorophore with 2,4-dinitrobenzene functional group. A kinetic study of TPP towards thiophenol displayed a fast response time (~150s) and significant turn-on fluorescence enhancement (~60 fold). Selective and competitive experiments exhibited an excellent selectivity of TPP toward thiophenols over biothiols (Cys, GSH) and other aliphatic thiols or nucleophiles. Using this ultrasensitive probe (LOD, 1.05×10-8M), we have successfully monitored and quantified highly toxic thiophenols in aqueous media and real-water samples.
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