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Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation
Published on: November 26, 2018
Gene analysis of two cases with CisAB/B blood subgroup
Li-Xin Jiao1, Jiang-Hong Yu1, Xiao-Li Yu1
1Blood Center of Jilin Province, Changchun 130033, China.
Objective:
To identify the precise ABO blood type subgroups with genotyping.
Method:
We screened ABO blood type from voluntary blood donors in our blood collection center. Samples were first examined with a routine serological method. Samples with ambiguous results were further examined with genotyping to identify ABO subgroups.
Results:
Two samples identified as AweakB by serology were considered to be A/B and B/B by PCR-SSP. However, the gene sequencing results revealed the precise subgroup to be CisAB01/B101 and CisAB05/B101, respectively.
Conclusion:
It may be difficult to identify non-typical AB patients with a routine serological method. Genotyping is a more precise method to identify blood subgroups.
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