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Toward a Complete North American Borrelia miyamotoi Genome

Luke C Kingry1, Adam Replogle1, Dhwani Batra2

  • 1Division of Vector-Borne Diseases, Bacterial Diseases Branch, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA.

Genome Announcements
|February 4, 2017
PubMed

Insights

Borrelia miyamotoi, a relapsing-fever spirochete, is an emerging tick-borne illness. This study sequences the genome of a Borrelia miyamotoi strain from a US tick, detailing its chromosome and plasmids.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Borrelia miyamotoi is an emerging tick-borne pathogen.
  • It belongs to the relapsing-fever spirochete group.
  • Human illness occurs in the Northern Hemisphere.

Purpose of the Study:

  • To present the genomic sequence of Borrelia miyamotoi.
  • To characterize the extrachromosomal plasmids of the bacterium.

Main Methods:

  • Whole-genome sequencing of Borrelia miyamotoi.
  • Analysis of chromosomal and extrachromosomal DNA elements.

Main Results:

  • The complete chromosome sequence was determined.
  • Eight extrachromosomal linear plasmids were identified.
  • Draft sequences for six additional plasmids (five circular, one linear) were obtained.

Conclusions:

  • Genomic data provides a foundation for understanding Borrelia miyamotoi.
  • Characterization of plasmids is crucial for studying its biology and pathogenicity.
  • This work contributes to the surveillance of tick-borne diseases.

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