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Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

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Related Experiment Video

Updated: Mar 8, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

Fluorescence Imaging for Cancer Screening and Surveillance.

K E Tipirneni¹, E L Rosenthal², L S Moore³

¹Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA.

Molecular Imaging and Biology

|February 4, 2017

Summary

This summary is machine-generated.

Fluorescence imaging (FI) shows promise for early cancer detection and surgical guidance. This technology aids in identifying lesions missed by conventional methods, improving patient outcomes.

Keywords:

Cancer screening Diagnostic imaging Early detection of cancer Molecular imaging Neoplasms Optical imaging

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Area of Science:

Oncology
Medical Imaging
Biotechnology

Background:

Fluorescence imaging (FI) is emerging as a key technology in oncology.
Early cancer detection and effective surgical resection are critical for improving patient survival rates.
Current imaging modalities have limitations in detecting subtle or early-stage cancerous lesions.

Purpose of the Study:

To review the current applications of fluorescence imaging in cancer screening and detection.
To highlight the potential of FI in identifying lesions not visible with conventional methods.
To discuss the role of FI in managing high-risk patients and those under surveillance.

Main Methods:

Review of existing literature and clinical trials on fluorescence imaging in oncology.
Analysis of FI's utility across various cancer types for screening and detection.
Evaluation of FI's potential to address healthcare disparities and the global cancer burden.

Main Results:

FI demonstrates significant promise for enhancing cancer screening and surveillance.
The technology aids in the early detection of lesions, potentially improving surgical outcomes.
FI offers a cost-effective imaging solution, addressing global healthcare needs.

Conclusions:

Fluorescence imaging is a valuable tool for early cancer detection and improved surgical resection.
FI has the potential to revolutionize cancer care by enabling earlier diagnosis and treatment.
Further clinical evaluation is ongoing to establish FI's widespread utility in oncology.