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Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary
Vittorio Bertone1, Eleonora Tarantola2, Isabel Freitas2
1Department of Biology and Biotechnology "Lazzaro Spallanzani", University of Pavia, via A. Ferrata, 9, 27100, Pavia, Italy. bertone@unipv.it.
Methods in Molecular Biology (Clifton, N.J.)
|February 4, 2017
Abstract:
Dipeptidyl-peptidase IV is an enzyme involved in a lot of biochemical processes, where it modifies a number of regulatory proteins by removing the terminal peptides by hydrolysis. Here we describe a histochemical method to demonstrate with accuracy and precision its in situ activity on cryostatic section of Wistar rat liver by means of a simultaneous azo-coupling method.
