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Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses
Published on: October 7, 2020
The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium
Vicente Seco-Rovira1, Ester Beltrán-Frutos1, Jesús Martínez-Hernández1
1Department of Cell Biology and Histology, School of Medicine, IMIB-Arrixaca, University of Murcia, Regional Campus of International Excellence, Campus Mare Nostrum, Espinardo, Murcia, 30100, Spain.
Abstract:
Lectin histochemistry is commonly used to characterize the pattern of glycoconjugates in cells and tissues. Recent studies show that alterations in these glycoconjugates are associated with the entry of cells into apoptosis. A widely used technique for the detection of apoptotic cell death is TUNEL. In this chapter, we study the sensitivity of both techniques to identify apoptotic cells in the testis of photo-inhibited Syrian hamster.
