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[The polypeptides of mumps virus and their synthesis in infected chick embryo cells]
Abstract:
Strain ME of mumps grown in chick amniotic cavity and purified by differential centrifugation combined with sucrose gradient centrifugation. The structural polypeptides of purified virion were analyzed by SDS polyacrylamide gel electrophoresis. Eleven polypeptides were found. Their molecular weight were between 35K-72K dalton. In addition polymers of HN protein and F1 the large subunit of F protein were detected. Hela, Vero and CE cells were infected with ME strain of mumps virus. The CE cell reveals the most sensitive host cell. CE cell infected with mumps virus and labeled with [35S]-Met, SDS-PAGE and examined by autoradiography. It was found at least eight polypeptides were synthesis in host cells and their molecular weight were between 26.5K to 94K dalton. The time course of the synthesis of polypeptides in the cells were studies. Palse-Chase experiments demonstrated the posttranslational process of Fo----F.
Insights
This study characterizes mumps virus (ME strain) structural polypeptides and host cell protein synthesis. Chick embryo (CE) cells are identified as the most sensitive host for mumps virus infection and polypeptide analysis.
Area of Science:
- Virology
- Molecular Biology
- Biochemistry
Context:
- Mumps virus structural proteins are crucial for understanding viral assembly and pathogenesis.
- Previous characterization of mumps virus polypeptides and their synthesis in host cells requires further detailed analysis.
Purpose:
- To identify and characterize the structural polypeptides of the mumps virus (ME strain).
- To investigate the synthesis of viral polypeptides in different host cells.
- To determine the most sensitive host cell for mumps virus infection and polypeptide analysis.
Summary:
- The mumps virus (ME strain) was purified, and its structural polypeptides were analyzed using SDS-PAGE, revealing eleven polypeptides (35K-72K daltons) and polymers of HN and F1 proteins.
- Infection of Hela, Vero, and chick embryo (CE) cells showed CE cells to be the most sensitive.
- Analysis of [35S]-Met labeled CE cells infected with mumps virus identified at least eight synthesized polypeptides (26.5K-94K daltons) and demonstrated the posttranslational processing of F protein via pulse-chase experiments.
Impact:
- Provides a detailed molecular characterization of mumps virus structural components.
- Identifies chick embryo cells as a highly sensitive model for mumps virus research.
- Elucidates the synthesis and posttranslational modification of mumps virus proteins, contributing to vaccine development and antiviral strategies.