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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

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Long noncoding RNA RP11-766N7.4 functions as a tumor suppressor by regulating epithelial-mesenchymal transition in

Guo-Liang Yao1, Chun-Feng Pan1, Hairong Xu2

  • 1Department of Thoracic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, PR China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|February 4, 2017
PubMed
Abstract

Insights

Long non-coding RNA RP11-766N7.4 is downregulated in esophageal squamous cell carcinoma (ESCC), acting as a tumor suppressor. Its expression correlates with patient survival, indicating potential as a prognostic marker and therapeutic target for ESCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in various cancers, including esophageal squamous cell carcinoma (ESCC).
  • The specific role of the novel lncRNA RP11-766N7.4 in ESCC oncogenesis and metastasis was previously unknown.

Purpose of the Study:

  • To investigate the functional role of lncRNA RP11-766N7.4 in the development and progression of ESCC.
  • To evaluate lncRNA RP11-766N7.4 as a potential prognostic biomarker for ESCC patients.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) to assess lncRNA RP11-766N7.4 expression in ESCC cell lines and tissues.
  • Correlation analysis of expression levels with clinicopathological features and patient survival.
  • In vitro functional assays including proliferation, cell cycle, apoptosis, migration, and invasion assays.
  • Western blotting to explore underlying regulatory mechanisms, including epithelial-mesenchymal transition (EMT).

Main Results:

  • lncRNA RP11-766N7.4 was significantly downregulated in ESCC tissues and cell lines.
  • Lower expression of lncRNA RP11-766N7.4 correlated with advanced tumor stage, lymph node metastasis, and reduced patient survival.
  • Knockdown of lncRNA RP11-766N7.4 promoted ESCC cell migration and invasion by inducing EMT, while its overexpression inhibited these processes by suppressing EMT.
  • lncRNA RP11-766N7.4 did not significantly affect cell proliferation, cell cycle, or apoptosis.

Conclusions:

  • lncRNA RP11-766N7.4 functions as a tumor suppressor in ESCC.
  • The lncRNA RP11-766N7.4 pathway is involved in regulating ESCC cell metastasis via the EMT process.
  • lncRNA RP11-766N7.4 represents a potential prognostic marker and therapeutic target for esophageal squamous cell carcinoma.
Keywords:
EMTESCCLong non-coding RNAMetastasisRP11-766N7.4

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