Related Experiment Video
Updated: Mar 8, 2026

Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
Published on: August 9, 2019
Theoretical algorithm and application of a double-integrating sphere system for measuring leaf transmittance and
Abstract:
We present the theoretical background and analytical equations for calculating spectral reflectance and transmittance factors for plant leaves from data collected by a field spectroradiometer attached to a double-integrating sphere. The sphere constants required for the calculations are derived from measurements of an empty sample port and a diffuse reflectance panel. The new method is applied in measuring the spectra of leaves belonging to 13 tree species. The greatest advantages of a double-sphere system compared with the conventional single-sphere substitution method is the speed of measurements, ease of operation, and increased portability and field-ruggedness.
Related Concept Videos
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
UV–Vis Spectrometers
Light Acquisition
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview
The ATR process begins by directing a beam...
UV–Vis Spectroscopy: Beer–Lambert Law
The Antenna Complex

