Engineered nanoparticles of titanium dioxide (TIO₂): Uptake and biological effects in a sea bass cell line

With the rapid development of nanotechnology there has been a corresponding increase in the application of titanium dioxide nanoparticles (TiO₂-NPs) in various consumer and industrial products, consequently their potential health hazards and environmental effects are considered an aspect of great concern. In the present study, in order to assess the impact of TiO₂-NPs in the marine environment, the biological effects of TiO₂-NPs on a sea bass cell line (DLEC) were investigated. Cells were exposed for 24 h to different concentrations of TiO₂-NPs (1, 8, 40, 200 and 1000 μg/ml) or co-exposed with CdCl₂ (Cd). The effects of UV light irradiation were also investigated in cells treated with TiO₂-NPs and/or Cd. The internalization of TiO₂-NPs and the morphological cell modifications induced by the treatments were examined by transmission and scanning electron microscopy, this latter coupled with energy dispersive X-ray spectroscopy (EDS) for particle element detection. In addition, the effects of controlled exposures were studied evaluating the cytotoxicity, the DNA damage and the expression of inflammatory genes. Our study indicates that TiO₂-NPs were localized on the cell surface mainly as agglomerates revealed by EDS analysis and that they were uptaken by the cells inducing morphological changes. Photoactivation of TiO₂-NPs and/or co-exposure with Cd affects ATP levels and it contributes to induce acute cellular toxicity in DLEC cells dependent on Ti concentration. The inflammatory potential and the DNA damage, this latter displayed through a caspase-3 independent apoptotic process, were also demonstrated. Overall our data suggest that the interaction of TiO₂-NPs with marine water contaminants, such as cadmium, and the UV irradiation, may be an additional threat to marine organisms.