Related Experiment Video
Updated: Mar 8, 2026

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Synthesis, Characterization and Luminescence Sensitivity with Variance in pH, DNA and BSA Binding Studies of Ru(II)
Ravi Kumar Vuradi1, Srishailam Avudoddi1, Venkat Reddy Putta1
1Department of Chemistry, Osmania University, Hyderabad, Telangana State, 500007, India.
Abstract:
Three ruthenium(II) polypyridyl complexes, [Ru(phen)2(mip)](ClO4)2 (1) (phen =1,10-Phenanthroline), [Ru(bpy)2(mip)](ClO4)2 (2) (bpy = 2,2'bipyridyl) and [Ru(dmb)2(mip)](ClO4)2 (3) (dmb = 4, 4'-dimethyl 2, 2'-bipyridine), were synthesized with an intercalative ligand mip (2-morpholino-1H-imidazo[4,5-f][1, 10]phenanthroline) and characterized by 1H, 13C-NMR, IR, UV-vis, mass spectra and elemental analysis. pH effect, ion selectivity (cations, anions) and solvent sensitivity of complexes were studied. The interaction of these complexes with DNA was performed using absorption, emission spectroscopy and viscosity measurements. The experimental results indicated that the two complexes interacted with calf thymus DNA (CT-DNA) by intercalative mode. BSA (Bovine Serum Albumin) protein binding of these complexes was studied by UV-visible and fluorescence techniques. The binding capacity of these complexes was explained theoretically by molecular docking method.
More Related Videos
Related Concept Videos
Photoluminescence: Applications
Variables Affecting Phosphorescence and Fluorescence
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...

