Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

797
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
797
Methods of Classification and Identification01:28

Methods of Classification and Identification

1.5K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.5K
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

640
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
640

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Olanzapine Use and Vomiting Incidence in Chemotherapy-receiving Patients With Cervical Cancer: Multicenter Post Hoc Analysis.

In vivo (Athens, Greece)·2026
Same author

Development and exploratory evaluation of a prediction model for oral mucositis in patients receiving CapeOX chemotherapy: a multicenter retrospective study.

Scientific reports·2026
Same author

Association Between Brachytherapy and Acute Diarrhea During Chemoradiotherapy for Cervical Cancer: A Multicenter Retrospective Study.

Anticancer research·2026
Same author

Nutrition and Exercise Interventions During Hospitalization in Frail or Sarcopenic Patients: A Scoping Review of Intervention Configurations and Evidence Gaps.

Nutrients·2026
Same author

Association Between Time to First Mobilization and Recovery of Oral Intake Function in Patients with Pneumonia: A Two-Center Retrospective Cohort Study.

Life (Basel, Switzerland)·2026
Same author

Establishment of Irinotecan-Induced Oral Mucositis Hamster Model and Evaluation of Therapeutic Agents.

Biological & pharmaceutical bulletin·2026

Related Experiment Video

Updated: Mar 8, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
06:53

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Published on: May 5, 2023

4.3K

Molecular Diagnostic Techniques for Onychomycosis: Validity and Potential Application.

Shinichi Watanabe1, Kazuya Ishida2

  • 1Department of Dermatology, Teikyo University School of Medicine, 11-1. Kaga-2, Itabashi, Tokyo, 173-8605, Japan. watanabe@med.teikyo-u.ac.jp.

American Journal of Clinical Dermatology
|February 5, 2017
PubMed
Summary

Real-time polymerase chain reaction (PCR) offers a faster and more accurate method for diagnosing onychomycosis compared to traditional fungal cultures. This molecular technique significantly improves the detection rates of dermatophytes like Trichophyton species.

More Related Videos

Identification of Mycobacterium Species by DNA Microarray Chip Method
06:27

Identification of Mycobacterium Species by DNA Microarray Chip Method

Published on: June 24, 2025

693
Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
12:02

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Published on: May 2, 2018

13.1K

Related Experiment Videos

Last Updated: Mar 8, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
06:53

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

Published on: May 5, 2023

4.3K
Identification of Mycobacterium Species by DNA Microarray Chip Method
06:27

Identification of Mycobacterium Species by DNA Microarray Chip Method

Published on: June 24, 2025

693
Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
12:02

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Published on: May 2, 2018

13.1K

Area of Science:

  • Medical Mycology
  • Molecular Diagnostics
  • Dermatology

Background:

  • Onychomycosis diagnosis relies on microscopy and fungal culture.
  • Fungal culture methods are time-consuming and have limited sensitivity.
  • There is a need for more efficient diagnostic tools for nail fungal infections.

Purpose of the Study:

  • To compare the diagnostic performance of real-time PCR with conventional fungal culture for identifying dermatophytes in onychomycosis.
  • To validate a real-time PCR assay for detecting Trichophyton species in nail specimens.

Main Methods:

  • Nail specimens from 149 patients with confirmed onychomycosis were analyzed.
  • Real-time PCR assay was performed on all specimens.
  • Conventional fungal culture was used as a comparative method.

Main Results:

  • Real-time PCR identified dermatophytes in 95.3% of specimens, compared to 69.8% by fungal culture.
  • Complete concordance was observed for Trichophyton species between PCR and culture.
  • PCR provided results significantly faster (≤2 days vs. ≤28 days).

Conclusions:

  • Real-time PCR is a highly sensitive and rapid method for diagnosing onychomycosis.
  • The molecular method surpasses conventional culture in identifying causative dermatophytes.
  • Mixed infections with T. mentagrophytes and T. rubrum appear uncommon.