Mixotrophic cultivation of Chlorella for local protein production using agro-food by-products

A local strain of Chlorella vulgaris was cultivated by using cheese whey (CW), white wine lees (WL) and glycerol (Gly), coming from local agro-industrial activities, as C sources (2.2gCL^-1) to support algae production under mixotrophic conditions in Lombardy. In continuous mode, Chlorella increased biomass production compared with autotrophic conditions by 1.5-2 times, with the best results obtained for the CW substrate, i.e. 0.52gL^-1d^-1 of algal biomass vs. 0.24gL^-1d^-1 of algal biomass for autotrophic conditions, and protein content for both conditions adopted close to 500gkg^-1 DM. Mixotrophic conditions gave a much higher energy recovery efficiency (EF) than autotrophic conditions, i.e. organic carbon energy efficiency (EF_oc) of 32% and total energy efficiency (Ef_t) of 8%, respectively, suggesting the potential for the culture of algae as a sustainable practice to recover efficiently waste-C and a means of local protein production.