[Study on the metabolites of cui-xing-an in rat urine]

m-[(2-Dimethylamino) ethoxy] phenyl-N, N-dimethyl carbamate (Cui-Xing-An) is a new central anticholinesterase drug. After oral administration of Cui-Xing-An to rats, four components were isolated and purified from urine extract by HPLC. They were identified by MS to be the parent drug, m-[2-dimethylamino) ethoxy] phenyl-N-hydroxymethyl-N-methyl carbamate (hydroxylated Cui-Xing-An), m-[(2-dimethylamino) ethoxy] phenyl-N-methyl carbamate (N-demethylated Cui-Xing-An) and m-[(2-dimethylamino) ethoxy] phenol (hydrolysate). Experiment on Torpediniforms Nacline timilei AchE inhibitory rate showed that the N-demethyl Cui-Xing-An had the same anticholinesterase activity as the parent drug. The hydroxylated Cui-Xing-An exhibited lower activity, and the hydrolysate was inactive.