Human Papillomavirus Genotyping by Surface Plasmon Resonance-Based Test.

Area of Science:

• Biotechnology
• Medical Diagnostics
• Virology

Background:

• Human papillomavirus (HPV) infections are a significant global health concern.
• Accurate HPV genotyping is crucial for effective screening and management.
• Current diagnostic methods have limitations in terms of cost, speed, or throughput.

Purpose of the Study:

• To evaluate the performance of a surface plasmon resonance (SPR)-based test for human papillomavirus (HPV) genotyping.
• To assess the SPR test's utility as a screening method in a clinical setting in China.

Main Methods:

• The SPR test was compared against the hybrid capture II (HC2) test and direct DNA sequencing.
• Cervical specimens were collected from 424 patients diagnosed with chronic cervicitis or abnormal vaginal bleeding.
• Cytological and histological studies were also performed for comprehensive analysis.

Main Results:

• The SPR test demonstrated high concordance with both HC2 (overall 90.57%) and direct DNA sequencing (97.41%).
• Compared to DNA sequencing, the SPR test achieved a sensitivity of 96.86% and a specificity of 97.74%.
• The SPR test showed excellent positive, negative, and overall agreement with established methods.

Conclusions:

• The SPR test is a reliable, sensitive, and specific method for HPV genotyping.
• The SPR test offers advantages over hybrid-capture assays, including potential for simultaneous multi-specimen processing, lower cost, speed, and ease of use.
• The SPR test has significant potential for widespread adoption in HPV genotyping programs globally, particularly in China.