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Force-Induced H2S by PDLSCs Modifies Osteoclastic Activity during Tooth Movement
11 Department of Orthodontics, Peking University School and Hospital of Stomatology, Haidian District, Beijing, P.R. China.
Journal of Dental Research
|February 7, 2017
Summary
Mechanical force stimulates periodontal ligament stem cells (PDLSCs) to produce hydrogen sulfide (H2S). This gasotransmitter regulates osteoclast activity and tooth movement during orthodontic treatment.
Area of Science:
- Biochemistry
- Cell Biology
- Orthodontics
Background:
- Hydrogen sulfide (H2S), a gasotransmitter, influences mesenchymal stem cell (MSC) function and bone homeostasis.
- Periodontal ligament stem cells (PDLSCs) are key MSCs in the periodontal ligament, responding to mechanical forces during orthodontic tooth movement (OTM).
- The role of endogenous H2S production by PDLSCs in response to mechanical force and its impact on alveolar bone homeostasis remains unclear.
Purpose of the Study:
- To investigate whether mechanical force induces endogenous H2S production by PDLSCs.
- To determine if H2S regulates alveolar bone homeostasis and tooth movement during OTM.
- To elucidate the molecular mechanisms by which force-induced H2S influences osteoclastic activity.
Main Methods:
- Utilized a mouse OTM model to assess H2S production, macrophage accumulation, and osteoclastic activity in PDL tissue.
- Examined cystathionine β-synthase (CBS) expression and H2S production in PDLSCs under mechanical force.
- Manipulated endogenous H2S levels (blocking or increasing) to evaluate effects on force-induced osteoclastic activities and tooth movement.
- Analyzed the role of H2S in regulating monocyte chemoattractant protein-1 (MCP-1) secretion and the receptor activator of nuclear factor-κB ligand/osteoprotegerin (RANKL/OPG) system in PDLSCs.
Main Results:
- Orthodontic force induced endogenous H2S production in PDL tissue, correlating with macrophage accumulation and increased osteoclastic activity.
- Mechanical force stimulated CBS expression and H2S production in PDLSCs.
- Modulating H2S levels affected force-induced osteoclastic activities and controlled tooth movement.
- Force-induced H2S production by PDLSCs promoted MCP-1 secretion and RANKL/OPG system expression, influencing macrophage migration and osteoclast differentiation.
Conclusions:
- PDLSCs produce H2S in response to mechanical force, acting as a signal transducer.
- Force-induced H2S production in PDLSCs regulates osteoclastic activities in alveolar bone.
- The H2S-mediated regulation of OTM involves the MCP-1 secretion and RANKL/OPG system.
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