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Updated: Mar 7, 2026
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
Published on: December 12, 2011
Revitalizing genetically-modified mouse strains using frozen-thawed sperm after up to 192 h of refrigerated
Gonzalo Moreno-Del Val1, Patricia Muñoz-Robledano1
1Transgenic and Cryopreservation Laboratory, Servicio de Experimentación Animal UMH, Instituto de Neurociencias de Alicante, Consejo Superior de Investigaciones Científicas /Universidad Miguel Hernández, Alicante, Spain.
Abstract:
In the scientific interchange of genetically-modified mouse strains the transportation of refrigerated epididymis has several advantages over the transportation of live animals, especially with regard to the 3R (replacement, reduction and refinement) principles. The major limiting factor is the duration of the transportation. Previous reports have shown that sperm collected from transported epididymis maintained their fertility for around 72 h, but there are no published data with longer transportation times, and this window of time may be too short, especially for international shipments and where locations are not well connected. In this study live pups were born using frozen-thawed sperm after up to 192 h (8 days) of transportation, using a special in vitro fertilization design which resulted in a fertilization rate of 10.5%.
