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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

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Compact Quantum Dots for Single-molecule Imaging

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Published on: October 9, 2012

Nitrogen doped graphene quantum dots based long-persistent chemiluminescence system for ascorbic acid imaging.

Hongjun Chen¹, Qin Wang¹, Qinpeng Shen²

¹State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.

Biosensors & Bioelectronics

|February 8, 2017

Summary

A novel chemiluminescent imaging system using nitrogen-doped graphene quantum dots (NGQDs) and copper ions offers sensitive detection of ascorbic acid (AA) in fruit. This system provides a visual and stable tool for quantitative AA monitoring.

Keywords:

Ascorbic acid imaging Chemiluminescence Copper ion Nitrogen doped graphene quantum dots

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Area of Science:

Analytical Chemistry
Materials Science
Biomedical Imaging

Background:

Highly sensitive chemiluminescence (CL) imaging requires high photo-intensity and persistent emission.
Nitrogen-doped graphene quantum dots (NGQDs) are emerging as promising luminescent probes.
Ascorbic acid (AA) detection is crucial in food quality assessment.

Purpose of the Study:

To develop a copper ion-catalyzed long-persistent chemiluminescent imaging system for ascorbic acid detection.
To enhance the CL intensity and stability of NGQDs for imaging applications.
To create a visual sensor for quantitative AA monitoring in fruit.

Main Methods:

Fabrication of NGQDs as luminescent probes.
Utilizing H2O2 for direct oxidation and CL emission of NGQDs.
Employing Cu2+ ions to catalyze a Fenton-like reaction, significantly boosting CL intensity and persistence.
Development of a visual sensor based on the Cu2+/NGQDs/H2O2 system for AA detection.

Main Results:

Cu2+ ions enhanced NGQDs CL intensity by over 214 times via a catalyzed Fenton-like reaction.
The Cu2+/NGQDs system exhibited unique specificity against other metal ions.
A limit of detection (LOD) of 0.5 μM (S/N=3) was achieved for AA.
The system demonstrated high stability and proper sensitivity for AA detection in fruit.

Conclusions:

The developed Cu2+/NGQDs/H2O2 system provides a convenient and visual tool for AA determination.
The long-persistent chemiluminescent imaging method shows promising potential for sensitive and stable imaging sensing applications.
This approach offers a valuable method for quantitative AA monitoring in real fruit samples.