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Metschnikowia persici sp. nov., A Novel Protease-Producing Yeast Species from China
Chen Wang1,2, Yang Liu3, Tao-Tao Zhang2
1College of Food Science and Technology, Shi He Zi University, Xinjiang, 832003, China.
Abstract:
Three yeast strains, named as FHL-A, FHL-B, and FHL-C, were isolated from peach fruit surfaces collected from different regions in the North of China highly produced protease and were presented as single separate group in the genus Metschnikowia by sequence comparisons of 26S rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region. BLASTn alignments on NCBI showed that the similarity of 26S rRNA gene sequences of the three strains to all sequences of other yeasts accessed into the GenBank/EMBL/DDBJ and other database was very low (≦93%). The phylogenetic tree based on the D1/D2 region of 26S rRNA gene sequences revealed that three strains are most closely related to Metschnikowia koreensis KCTC 7828T (AF257272.1) (sequence similarity: 93.0%) and Metschnikowia reukaufii CBS9709T (AJ716113.1) (sequence similarity: 93.0%). However, the strains are distinguished from M. koreensis by its non-assimilation of galactose, ribitol, and D-xylose, and by its growth at 37 °C or in vitamin-free medium, and are notably different from M. reukaufii by its non-assimilation of galactose, D-xylose, D-arabinose, and D-ribose, and by its growth at 35 °C or in vitamin-free medium. The strain FHL-B formed asci in V8 juice sporulation medium for 3 weeks. Therefore, the name Metschnikowia persici is proposed for the novel species, with FHL-B (= CBS12815T = CFCC 3578T) as the type strain.
Insights
Three novel yeast strains, Metschnikowia persici, were isolated from Chinese peach fruits. These protease-producing yeasts are distinct from known Metschnikowia species based on genetic and physiological analyses.
Area of Science:
- Mycology
- Yeast Taxonomy
- Food Microbiology
Background:
- The genus Metschnikowia is known for yeasts isolated from various ecological niches, including plant surfaces.
- Protease-producing yeasts can have implications in food spoilage and industrial applications.
- Accurate taxonomic classification is crucial for understanding microbial diversity and function.
Purpose of the Study:
- To isolate and characterize novel yeast strains from peach fruit surfaces in China.
- To determine the taxonomic position of these strains within the genus Metschnikowia using molecular and physiological methods.
- To propose a new species name for distinct yeast isolates.
Main Methods:
- Isolation of yeast strains from peach fruit surfaces.
- Molecular identification using 26S rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region sequencing.
- Phylogenetic analysis based on sequence data.
- Physiological characterization including carbon assimilation tests and growth at different temperatures and in vitamin-free media.
Main Results:
- Three yeast strains (FHL-A, FHL-B, FHL-C) were isolated and found to produce protease.
- Sequence comparisons showed low similarity (≦93%) to known yeast species, indicating novelty.
- Phylogenetic analysis placed the strains close to Metschnikowia koreensis and Metschnikowia reukaufii but distinct based on physiological traits.
- Strain FHL-B formed asci, confirming sexual reproduction capabilities.
Conclusions:
- The isolated strains represent a novel species within the genus Metschnikowia.
- Metschnikowia persici sp. nov. is proposed, with strain FHL-B as the type strain.
- This finding expands the known diversity of Metschnikowia yeasts and their association with fruit surfaces.
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