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Serum phosphate concentration. Effect on serum ionized calcium concentration in vitro
1Department of Pediatrics, University of Cincinnati College of Medicine, OH 45267-0541.
American Journal of Diseases of Children (1960)
|November 1, 1989
Summary
Serum phosphate levels inversely affect serum ionized calcium concentrations. This study found that higher phosphate levels correlate with lower ionized calcium, independent of pH, highlighting phosphate
Area of Science:
- Biochemistry
- Clinical Chemistry
Background:
- Serum ionized calcium concentration is a critical physiological parameter.
- Factors like protein, bicarbonate, and pH are known to influence ionized calcium.
- The role of serum phosphate in modulating ionized calcium requires further elucidation.
Purpose of the Study:
- To investigate the in vitro effects of varying serum phosphate levels on serum ionized calcium concentrations.
- To determine the relationship between phosphate concentration, pH, and ionized calcium.
Main Methods:
- A single serum sample was divided into aliquots.
- pH was adjusted using hydrochloric acid or sodium hydroxide (range: 7.09–7.63).
- Phosphate concentration was altered using a phosphate buffer (range: 0.81–3.58 mmol/L).
Main Results:
- An inverse relationship was observed between ionized calcium and phosphate concentrations across all tested pH levels.
- Ionized calcium concentration showed a negative correlation with pH.
- Serum phosphate concentration independently influences serum ionized calcium levels.
Conclusions:
- Serum phosphate concentration is a significant factor affecting serum ionized calcium levels.
- In addition to pH, protein, and bicarbonate, phosphate should be considered when interpreting ionized calcium values.
- Further research is warranted to understand the clinical implications of this inverse relationship.